Journal: NPJ Regenerative Medicine

Article Title: Personalized medicine for reconstruction of critical-size bone defects – a translational approach with customizable vascularized bone tissue

doi: 10.1038/s41536-021-00158-8

Figure Lengend Snippet: a EPC after uptake of acetylated LDL-DiI (red) and lectin staining (green). Counterstaining with DAPI (blue). Scale bar 50 µm. b CD34 immunofluorescence staining (green) of EPC. Counterstaining with DAPI (blue). Scale bar 50 µm. c Sprouting of EPC spheroids after 48 h incubation in a fibrin gel. Scale bar 50 µm. d mRNA expression of EPC compared to MSC (set to 1). e Oil Red O staining of MSC after adipogenic differentiation. Scale bar 100 µm. f Alcian Blue staining of MSC after chondrogenic differentiation. Scale bar 200 µm. g Alizarin Red staining of MSC after osteogenic differentiation. Scale bar 200 µm.

Article Snippet: For the adipogenic differentiation, MSC were cultivated in a differentiation medium consisting of MSC adipogenic differentiation basal medium, supplemented with FCS, glutamine, insulin, 3-isobutyl-1-methyl-xanthine (IBMX), dexamethasone, rosiglitazone, and penicillin-streptomycin; for chondrogenic differentiation MSC were cultivated in MSC chondrogenic differentiation basal medium, supplemented with FCS, dexamethasone, ascorbate, sodium selenite (ITS), proline, TGF-β3, and penicillin-streptomycin; for osteogenic differentiation MSC were cultivated in MSC osteogenic differentiation basal medium, supplemented with FCS, glutamine, ascorbate, β-glycerophosphate, dexamethasone, and penicillin-streptomycin according to the manufacturer’s recommendation (all from Pelobiotech GmbH, Planegg, Germany).

Techniques: Staining, Immunofluorescence, Incubation, Expressing